Virus titrations using trypsin modified erythrocytes.

Detection of rubella haemagglutination-inhibition (HAI) and virus-specific IgM antibody using trypsin-treated human group O erythrocytes in the HAI test.

The modification of the standard rubella haemagglutination-inhibition (HAI) test using trypsin-treated human group O erythrocytes instead of chick erythrocytes was evaluated. In a comparative study we found that, of 816 samples tested by both methods, the titres of 807 (98.9%) sera were in close agreement within an acceptable twofold difference. Trypsin-treated human group O erythrocytes usuall...

Trypsin-Modified Human Erythrocytes

A method of treating human erythrocytes with trypsin has been modified and found to be an efficient and practical indicator system for the rubella hemagglutination-inhibition test. Both the trypsin-treated human cells and the widely used, newborn chicken erythrocytes were used in comparative testing of 464 selected diagnostic rubella serums. Results with each cell system were essentially the sa...

Preparation of Trypsin-treated Human Group 0 Erythrocytes

group 0 erythrocytes usually provided titres that were twofold higher than those obtained with chick erythrocytes. In general, a very good correlation between the two methods was obtained. Data are presented that emphasise the importance of trypsin treatment of human group 0 erythrocytes before use in the HAI method. Furthermore, we found that trypsin-treated human group 0 erythrocytes can be s...

The agglutination mechanism of trypsin modified red cells.

Transmembrane phospholipid motions induced by F glycoprotein in hemagglutinating virus of Japan.

Transfer of phospholipid from the envelope of hemagglutinating virus of Japan (HVJ) to erythrocyte (RBC) membrane and the virus-induced transfer of phospholipid between RBC membranes were studied using spin-labeled phosphatidylcholine (PC). The transfer of PC from membranes labeled densely with PC to unlabeled membranes was followed by the peak height increase in the electron spin resonance spe...